Historical Background
During 19th century the idea of development of callus (a disorganized proliferated mass of actively dividing cells) from isolated stem fragments and root apices came into existence. Callus could also be developed from buds, and root and shoot fragments of about 1.5 mm in size without using nutrient medium. For the first time in Berlin, Hamberlandt (1902) originated the concept of cell culture. He attempted to cultivate the isolated plant cells in vitro on an artificial medium (Knop's solution, peptone, asparagin and sucrose).
The term 'Tissue Culture' can be applied to any multicellular culture growing on a solid medium (or attached to substratum and nurtured with a liquid medium) that consists of many cells in protoplasmic continuity. But in organ culture (e.g. excised roots) the cultured plant material maintains its morphological identity, more or less, with the same anatomy and physiology as in vitro of the parent plants (Doods and Roberts, 1985).
Until the early 1930s, R.P. White (USA), Gautheret (France) and Nobercourt (France) independently cultured tissues excised from several plants on the defined nutrient media for a long period. Gautheret (1939) cultured cambium tissue of carrot on Knop's solution supplemented with other chemicals in trace amount. White (1939) cultured tobacco tumor tissue from the hybrid Nicotiana glduca, and N. Langsdorjfii.
In the 1950s several important achievements were made in the field of plant physiology. The understanding of plant growth hormone in rapid multiplication of totipotent cell was developed. At the university of Wisconsin, Skoog and co-workers found out the role of pytokinins in tissue culture. Consequently, several chemicals were tested which stimulated callus. Adenine in the presence of auxin was found to induce callus growth and bud formation in tobacco cultures (Skoog and Tsui, 1948). Eventually a potent cell division factor from degraded DNA preparations was isolated, identified and named as kinetin (Miller et al., 1955). The term cytokinin was given to this group after substituting the aminopurine compounds that stimulate cell division in cultured plant tissue and behave physiologically similar to kinetin. Later on other cytokinins (the naturally occurring plant hormones) such as zeatin, and isopentyl adenin were discovered. Skoog and Miller (1957) advanced the hypothesis of organogenesis in cultured callus by varying the ratio of auxin and cytokinin in the growth medium. The shoot was formed with keeping the ratio of kinetin higher and root developed when ratio was lower. |
